Structure-Activity Relationships in Diphtheria Toxin
نویسندگان
چکیده
منابع مشابه
Structure-activity relationships in diphtheria toxin.
The intact diphtheria toxin molecule, a single polypeptide chain of about 62,000 daltons, has no enzymic activity. However, the transfer in uifro of ADP-ribose from NAD to aminoacyltrensferase II can be catalyzed by any of several fragments of toxin. The smallest active fragment (A, 24,000 daltons) is normally connected to the remainder of the molecule (B, 38,000 daltons) by a peptide bond and ...
متن کاملCrystal structure of nucleotide-free diphtheria toxin.
The crystal structure of diphtheria toxin (DT) in the absence of nucleotide (nucleotide-free DT) has been determined at 2.3 A resolution to a crystallographic R factor and free R factor of 18.2 and 28.2%, respectively. A comparison of this structure to the previously determined structures of DT in complex with adenyly(3'-5')uridine monophosphate (ApUp) and DT in complex with nicotinamide adenin...
متن کاملDiphtheria toxin: mode of action and structure.
INTRODUCTION.... 54 OUTLINE OF THEPROBLEM. 54 INHIBITION OFPROTEIN SYNTHESIS ..56 Studies in Cell Culture ..56 Studies in Cell-Free Systems . 8 ADP-RIBOSYLATION OF EF-2 .59 Identification of the Reaction . ADPR is Covalently Attached . .60 The Role of Toxin is Catalytic . 1 Substrate Specificity ..6 Reversibility and Thermodynamics .62 Factors Affecting the Rate of the Forward Reaction 62 STRUC...
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It has been demonstrated that a saturating dose of diphtheria toxin produced a 90% inhibition of polio-virus replication in HeLa cells. This inhibition was reflected in infectious viral RNA synthesis and in mature virus production. Toxin had no direct effect on virus particles or I-RNA, and poliovirus adsorption and eclipse appeared to be carried out normally in intoxicated cells. When toxin wa...
متن کاملAntigenic relationships on the diphtheria toxin molecule: antitoxin versus antitoxoid.
We used the mouse to produce antisera to native diphtheria toxin and diphtheria toxoid. With these antisera it was possible to distinguish between toxin and toxoid. By gel diffusion analysis, antitoxin detected antigenic determinants on toxin which were not available on toxoid, indicating that some determinants had been lost or altered by formalin treatment. Antitoxoid, on the other hand, showe...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1971
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)76999-3